Multiplex ELISA Service

Comparing with traditional ELISA kit can only be used to detect one biomarker, new generation of Multiplex technique includes the advantages of both ELISA and Array system for detecting multiple Biomarkers with a small amount of sample. The Multiplex service of Biotools SuperLab, providing accurate high-throughput data for multiple protein biomarker quantifications with Multiplex ELISA system of Bosterbio. Meanwhile, we can assist customers to build this user-friendly and cost-effective system in laboratory.

Features of Services:
1. High-throughput data, simultaneously quantifying multiple protein biomarkers
2. Save sample volume
3. Save time
4. Cost-effective
5. Processing by our professional scientists
Multiplex ELISA technique is based on the concept of Microarray, coating droplet of multiple capture antibodies on the bottom of well. Luminol-based chemiluminescent substrate and Horseradish peroxidase (HRP) are used to produced chemiluminescent signal for detection. We recommend that using Q-View Imager or Fluorescent scanner (LI-COR Odyssey) as detector to capture the chemiluminescent results, these high-resolution detectors can perfectly provide high accurate data for analysis. Our professional scientists are using Q-View Imager to process the Multiplex service, we are confident that our services are able to meet your needs.
Comparing with traditional ELISA, Multiplex ELISA can not only bring down the cost of high-throughput determination of sample, which can also greatly reduce the experimental error of multiple biomarker determinations by detecting them together in one kit.
Advantages of Multiplex ELISA:
  1. Low sample volume (Loading with only 50 µl sample per well)
  2. High sensitivity (Lower Limit of Detection reaches around 4 pg/ml)
  3. Flexible in detection methods (Combining two ELISA methods in one plate, like Sandwich and Competitive ELISA methods)
  4. High efficiency (Simultaneously identifying multiple targets together)
  5. Customization (Many analytes for choosing)
  Traditional ELISA Kit Multiplex ELISA Kit
Sample volume 100-200ul 50ul
Targets 1 plex/plate 2-18 plex/plate
Processing time 4 hours 3 hours
Sensitivity High High
Detection system TMB Chemiluminescent

Ready-to-Use Multiplex ELISA Kits:
Cat. No. Species Panel Analytes
MEK1001 Human Cytokine IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10 IL-12p70, IL-13, IL-15, IL-17, IL-23, IFNγ, TNFα, TNFβ
MEK1016 Mouse Cytokine IFN-γ, IL-1β, IL-6, TNFα
MEK1018 Porcine Cytokine IL-1β, IL-6, IL-8, TNFα
MEK1019 Human Chemokine Eotaxin, GROa, I-309, IL-8, IP-10, MCP-1, MCP-2, Rantes, TARC
MEK1021 Human Angiogenesis Ang-2, FGF basic, HGF, IL-8, PDGF-BB, TIMP-1, TIMP-2, TNFα, VEGF
MEK1022 Human Female Hormone Adiponectin, Cortisol, C-Peptide, E1G, FSH, HCGb, IL-6, IL-10
MEK1023 Human MMP MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13
MEK1024 Human Micronutrient AGP, CRP, Ferritin, sTfR, RBP4, Tg, HRP2
MEK1025 Rat Inflammation IFN-γ, IL-1α, IL-1β, IL-2, IL-4, IL-6, IL-10, IL-12p70, TNFα
More choice on Boster Biological Technology's website:

「Principle of Multiplex ELISA」
Multiplex ELISA kits are including high specific antibodies, which are accurately printed at the corresponding positions on the surface of each well by . The high quality control standards of Multiplex ELISA kits are determined by specialized team of Boster Biological Technology, confirming the printing accuracy, differentiating each specific antibody spot on a well, identifying the excellent results of the kits.
Using Multiplex ELISA kits, loading with only 50 µl of sample to a well. The targets in the sample are completely captured by the corresponding high specific antibody spots in the well, the streptavidin-HRP will be used to generate chemiluminescent signal for quantification of each target. There are 18 targets (maximum) can be quantified together in a well of Multiplex ELISA kits with high sensitive chemiluminescence detection system, the LLOD (Lower Limit of Detection) of most target is optimized at high sensitive level (pg/ml).
「Professional Multiplex ELISA services of BioTools」
The results of Multiplex ELISA kits are precisely determined by using Q-View Imager system, which provides high efficiency, high sensitivity, high resolution, and high throughput data. The chemiluminescent intensity of high throughput data is perfectly captured by Q-View Imager system in 5 minutes, and the data are analyzed using Q-View software.
Working time: 1-2 weeks (depending on the sample size)
1. Choosing the suitable kit (Boster - Multiplex ELISA Kit)
2. Providing enough sample volume for detection (ex. At least 150 µl of sample for 50 µl/well with two biological repeat)
3. Our service is not available for infectious organisms and whole blood.
1. Service report
2. Regression curve of services are determined by Q-View