Two-dimensional gel electrophoresis (2D-PAGE)

Two-dimensional gel electrophoresis is combined with isoelectric focusing (IEF) and SDS-PAGE methods to enhance the resolution of the electrophoresis.
The separation of different proteins with two-dimensional gel electrophoresis is based on the isoelectric point and the molecular point of different proteins. With two different separating methods, we are able to identify and quantify thousands of proteins.
We use IEF to perform one-dimension gel electrophoresis and separate the proteins according to their PI. Then we use SDS-PAGE to perform the two-dimension gel electrophoresis and separate the proteins according to their molecular weight.
With this method, we can identify the protein composition in a complicated sample, or analyze the difference of protein composition with different treatments.

Staining method: 
1. Coomassie blue stain
2. Silver stain

Working day not including delivery time: 3 weeks

(a) Sample type: Cell pellet、Protein extracts
(b) Sample volume: ≤150 uL
(c) Sample concentration: ≥1 ug/uL (Silver stain), ≥2 ug/uL (Coomassie blue stain)
(d) Platform: Ettan IPGphor 3 IEF system/Hoefer SE600

Please click the link below for the specification of Proteomics service

Sample requirement
1. Summary report. pdf
Q1. What is the size of the gel to perform IEF and SDS-PAGE?
A: For IEF we use the gel length of 13 cm. For SDS-PAGE we use the gel size of13 cm x 13 cm x 1.5 mm.
Q2. Do you have staining service?
A: Yes, we provide Coomassie blue stain and Silver stain。
Q3. Do you provide visual analysis of the gel?
A: Due to the limitation of the software, we can not provide this service.

Q4. Do you have the image analysis service?
A: No, we don't. We don't have the relative instrument and software for image analysis.

Q5.The specification of IEF and SDS-PAGE?
A: IEF: 13 cm;  SDS-PAGE: 13cmx13cmx1.5mm

Q6. What type of staining method do you have?
A: We provide coomassie blue staining and silver staining.