Epitope mapping (HDX)
interaction or antibody-protein interaction site.
Working day not including delivery time: 5 weeks
(a) Sample type: Purified protein with amino acid sequence and condition of molecules interaction
(b) Sample amount: ≥200 pmol (~10 ug for 50 kDa protein)
(c) Sample concentration: ≥4.5 pmol/uL (~0.23 ug/uL for 50 kDa protein)
(d) Sample purity: ≥90%
(e) Platform: online-Pepsin-LC, Orbitrap HDX-MS
Please click the link below for the specification of Proteomics service
1. Summary report. pdf
2. Protein-peptide report. xlsx
Q2. Is it possible for me to identify protein-drug or protein-DNA interaction site?
A: Of course, just provide us the condition of the interaction, and we will evaluate the possibility.
Q3. Can HDX used in identifying the binding region of protein and nucleic acid?
A: Yes, if you can provide the binding condition of the nucleic acid and protein, unless the DNA:Protein ratio is too high to effect the signal of spectormetry (<1:100, molar ratio, the closer the better). The purity of protein must be >90%)
Q4. Do you provide protein-protein binding condition test?
A: No, we do not provide this service.
Q5. What is the application of HDX besides analyzing the binding site of protein and antibody?
A: HDX can also analyze the binding site of protein and drugs, or the site of protein coformational change.