rno-miR-184

TOOL miRNA RT-qPCR primer/probe set is an integral part of TOOL miRNA RT-qPCR assay which is based on two-step RT-qPCR to especially target microRNAs. Each TOOLS miRNA RT-qPCR primer/probe set is packaged including two parts: one tube containing the unique designed stem-loop RT primer and the other containing a mix of the qPCR probe, forward, and reverse primers. 
No. Size Price Qty
TTH-R-184 50 rxns. $250.00
TTH-R-184 250 rxns $850.00
The price does not include shipping fee and tax. ORDER
Features
  • Comprehensive: quantitate only mature miRNAs, not precursors
  • Sensitive: sequence-specific primers
  • ​Efficient, simple, and scalable: two-step quantitative RT-PCR assay provides high-quality results in less than three hours


Assay information
miRBase Accession Number
MIMAT0000861
Chromosome Location
chr8:97175666-97175687(-)
Mature miRNA Sequence:
UGGACGGAGAACUGAUAAGGGU
Species:
Rattus norvegicus
Assay System:
Probe-base qPCR assay
 


Design of TOOLS miRNA RT-qPCR assay system
Product name miRNA ACC
rno-let-7a-5p MIMAT0000774
rno-let-7b-5p MIMAT0000775
rno-let-7c-5p MIMAT0000776
rno-let-7d-3p MIMAT0000563
rno-let-7d-5p MIMAT0000562
rno-let-7f-5p MIMAT0000778
rno-let-7g-5p MIMAT0035719
rno-let-7i-3p MIMAT0004707
rno-miR-1b MIMAT0037263
rno-miR-9a-3p MIMAT0004708
rno-miR-9a-5p MIMAT0000781
rno-miR-10a-3p MIMAT0004709
rno-miR-10a-5p MIMAT0000782
rno-miR-15b-3p MIMAT0017093
rno-miR-15b-5p MIMAT0000784
rno-miR-16-5p MIMAT0000785
rno-miR-17-5p MIMAT0000786
rno-miR-18a-5p MIMAT0000787
rno-miR-19a-3p MIMAT0000789
rno-miR-19b-3p MIMAT0000788
rno-miR-20a-5p MIMAT0000602
rno-miR-21-5p MIMAT0000790
rno-miR-22-3p MIMAT0000791
rno-miR-22-5p MIMAT0003152
rno-miR-24-3p MIMAT0000794
rno-miR-23a-3p MIMAT0000792
rno-miR-25-3p MIMAT0000795
rno-miR-26a-5p MIMAT0000796
rno-miR-26b-5p MIMAT0000797
rno-miR-27a-3p MIMAT0000799
rno-miR-27b-3p MIMAT0000798
rno-miR-29a-3p MIMAT0000802
rno-miR-29b-3p MIMAT0000801
rno-miR-29b-5p MIMAT0004717
rno-miR-30a-3p MIMAT0000809
rno-miR-30a-5p MIMAT0000808
rno-miR-30b-5p MIMAT0000806
rno-miR-30c-5p MIMAT0000804
rno-miR-30d-5p MIMAT0000807
rno-miR-30e-3p MIMAT0004720
Product name miRNA ACC
rno-miR-30e-5p MIMAT0000805
rno-miR-32-5p MIMAT0000811
rno-miR-33-5p MIMAT0000812
rno-miR-34a-5p MIMAT0000815
rno-miR-34c-5p MIMAT0000814
rno-miR-92b-3p MIMAT0005340
rno-miR-93-5p MIMAT0000817
rno-miR-98-5p MIMAT0000819
rno-miR-99a-5p MIMAT0000820
rno-miR-99b-5p MIMAT0000821
rno-miR-100-5p MIMAT0000822
rno-miR-101a-3p MIMAT0000823
rno-miR-103-3p MIMAT0000824
rno-miR-106b-3p MIMAT0004727
rno-miR-106b-5p MIMAT0000825
rno-miR-107-3p MIMAT0000826
rno-miR-122-5p MIMAT0000827
rno-miR-125a-5p MIMAT0000829
rno-miR-125b-5p MIMAT0000830
rno-miR-126a-3p MIMAT0000832
rno-miR-126a-5p MIMAT0000831
rno-miR-127-3p MIMAT0000833
no-miR-128-3p MIMAT0000834
rno-miR-130b-3p MIMAT0000837
rno-miR-132-3p MIMAT0000838
rno-miR-133a-3p MIMAT0000839
rno-miR-133a-5p MIMAT0017124
rno-miR-133b-3p MIMAT0003126
rno-miR-135a-5p MIMAT0000841
rno-miR-135b-5p MIMAT0000611
rno-miR-140-3p MIMAT0000574
rno-miR-141-3p MIMAT0000846
rno-miR-142-3p MIMAT0000848
rno-miR-142-5p MIMAT0000847
rno-miR-144-3p MIMAT0000850
rno-miR-145-5p MIMAT0000851
rno-miR-146a-5p MIMAT0000852
rno-miR-148b-3p MIMAT0000579
rno-miR-149-5p MIMAT0035726
rno-miR-150-5p MIMAT0000853
Product name miRNA ACC
rno-miR-181a-1-3p MIMAT0000884
rno-miR-181a-5p MIMAT0000858
rno-miR-181b-5p MIMAT0000859
rno-miR-183-5p MIMAT0000860
rno-miR-184 MIMAT0000861
rno-miR-185-5p MIMAT0000862
rno-miR-186-5p MIMAT0000863
rno-miR-187-3p MIMAT0000864
rno-miR-191a-5p MIMAT0000866
rno-miR-192-5p MIMAT0000867
rno-miR-194-5p MIMAT0000869
rno-miR-195-5p MIMAT0000870
rno-miR-196a-5p MIMAT0000871
rno-miR-196b-5p MIMAT0001082
rno-miR-199a-3p MIMAT0004738
rno-miR-200a-3p MIMAT0000874
rno-miR-203a-3p MIMAT0000876
rno-miR-204-5p MIMAT0000877
rno-miR-210-3p MIMAT0000881
rno-miR-218a-5p MIMAT0000888
rno-miR-221-3p MIMAT0000890
rno-miR-222-3p MIMAT0000891
rno-miR-296-3p MIMAT0004742
rno-miR-320-3p MIMAT0000903
rno-miR-328a-3p MIMAT0000564
rno-miR-335 MIMAT0000575
rno-miR-340-5p MIMAT0004650
rno-miR-342-3p MIMAT0000589
rno-miR-365-3p MIMAT0001549
rno-miR-374-5p MIMAT0003208
rno-miR-375-3p MIMAT0005307
rno-miR-378a-5p MIMAT0003378
rno-miR-423-3p MIMAT0005313
rno-miR-425-5p MIMAT0005314
rno-miR-451-5p MIMAT0001633
rno-miR-455-5p MIMAT0005316
rno-miR-499-5p MIMAT0003381
rno-miR-877 MIMAT0005285
rno-miR-1247-5p MIMAT0035721
TOOLS miRNA RT Kit
Cat#TTH-mi50 / TTH-mi250

TOOLS miRNA RT Kit provides the perfect first step of TOOLS miRNA RT-qPCR assay. This Kit adopts an innovative RT enzyme and buffer system, which enables the RT reaction completed within only 15 mins, and provides reliable reverse transcription to a wide range (from 15 pg to 150 ng) of RNA template.
  • Easy: Compatible with TOOLS miRNA RTqPCR primer/probe set, only common RT procedure required. 
  • Fast:  RT reaction completed only within 15 mins.
  • Accurate: Combination of an efficient reverse transcriptase and RNase inhibitor
  • Efficient: Reliable reverse transcription to a wide range (from 15 pg to 150 ng) of RNA template

TOOLS Easy 2xProbe qPCR Mix
Cat#TTC-QE13

This optimized ready-to-load mix contains dNTP/dUTP mix, Mg2+, modified hot-start DNA polymerase, dUTP/UDG anticontamination system, specific ROX Reference Dye, and all components necessary to be directly used for a robust and low-template qPCR with high sensitivity, specificity, and reliability. The dUTP/UDG anticontamination system included into the mix eliminates the product contamination of qPCR reactions.  TOOLS Easy 2×Probe qPCR Mix also contains ROX Reference Dye suitable for all qPCR instruments, and no adjustments are required for the dye concentration in different instruments.
  • Easy: All-in-one optimized master mix
  • Specific: Premixed with dUTP/UDG anticontamination system
  • High efficiency and stability
  • ROX Reference Dye for fluorescent signal normalization and compatible with all qPCR instruments.

EasyPrep miRNA Extraction Kit
Cat#DPT-BE01

EasyPrep miRNA Extraction Kit utilizes a silica-based system to enrich and purify small RNA, including miRNA, small interfering RNA (siRNA), small nuclear RNA (snRNA), and total RNA from various sample sources (cell, animal tissue, plant tissue, serum, plasma), especially for small RNA of <200 nt. The lysis buffer in the kit is optimized to exert excellent lysis ability and isolation efficiency. High-quality isolated product without contamination of DNA and protein could be obtained within 1 hour, and directly used in Northern Blot, Dot Blot, Poly A screening, in vitro translation, RNase protection analysis, and also molecular cloning.
  • Efficient: Completed within 1 hr
  • Quality: silica-based system to yield high-purity small RNA product
  • Flexible: Excellent lysis ability for various sample sources