「Principle of Multiplex ELISA」
Multiplex ELISA kits are including high specific antibodies, which are accurately printed at the corresponding positions on the surface of each well by . The high quality control standards of Multiplex ELISA kits are determined by specialized team of Boster Biological Technology, confirming the printing accuracy, differentiating each specific antibody spot on a well, identifying the excellent results of the kits. Comparing with traditional ELISA kit can only be used to detect one biomarker, new generation of Multiplex technique includes the advantages of both ELISA and Array system for detecting multiple Biomarkers with a small amount of sample. Using Multiplex ELISA kits, loading with only 50 µl of sample to a well. The targets in the sample are completely captured by the corresponding high specific antibody spots in the well, the streptavidin-HRP will be used to generate chemiluminescent signal for quantification of each target. There are 18 targets (maximum) can be quantified together in a well of Multiplex ELISA kits with high sensitive chemiluminescence detection system, the LLOD (Lower Limit of Detection) of most target is optimized at high sensitive level (pg/ml). 「Professional Multiplex ELISA services of BioTools」
The results of Multiplex ELISA kits are precisely determined by using Q-View Imager system, which provides high efficiency, high sensitivity, high resolution, and high throughput data. The chemiluminescent intensity of high throughput data is perfectly captured by Q-View Imager system in 5 minutes, and the data are analyzed using Q-View software. Comparing with traditional ELISA, Multiplex ELISA can not only bring down the cost of high-throughput determination of sample, which can also greatly reduce the experimental error of multiple biomarker determinations by detecting them together in one kit.