2x Ultra Hi-Fi PCR Master Mix

TOOLS 2× Ultra Hi-Fi PCR Master Mix is is a new generation superior enzyme based on Ultra Hi-Fi DNA Polymerase for robust PCR with higher fidelity. The unique extension factor, specificity-promoting factors and plateau-inhibiting factor newly added to Ultra Hi-Fi greatly improve its long-fragment amplification ability, specificity, and PCR yield. Ultra Hi-Fi is capable of amplifying long fragments such as 40 kb λ DNA, 40 kb plasmid DNA, 20 kb genomic DNA and 10 kb cDNA. The amplification error rate of P Ultra Hi-Fi is 53-fold lower than that of conventional Taq and 6-fold lower than that of Pfu. In addition, Ultra Hi-Fi has a good resistance to PCR inhibitors and can be used for direct PCR amplifications of bacteria, fungi, plant tissues, animal tissues, and even whole blood samples. P Ultra Hi-Fi contains two monoclonal antibodies inhibiting the 5 ́→3 ́ polymerase activity and 3 ́→5 ́ exonuclease activity at room temperature, which enable Ultra Hi-Fi to perform greatly-specific Hot-Start PCRs. 2× Ultra Hi-Fi PCR MasterMix contains 2× Ultra Hi-Fi Super-Fidelity DNA Polymerase, dNTP, and an optimized buffer system. The amplification can start only with the addition of primer and template, thereby easing PCR setup and improving reproducibility. Protective agents in this product are capable of repeated freeze-thaw cycles.
No. Size Price Qty
TTC-PE15 1ml $172.42
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  • High Fidelity: The fidelity is 53-fold higher than Taq DNA polymerase
  • Can amplify up to 40kb  
  • Can tolerate GC rich template
  • Can be used for direct PCR amplification
  • Fast: 2kb/sec
Ultra Hi-Fi PCR MasterMix Fidelity Test: 


Ultra Hi-Fi Competition Test:

Enzyme Speed Test:

Ultra Hi-Fi Fragment Extension Test:

Q1. Why is the length generally shorter for cDNA template?
A:cDNA has more complexed secondary structure. Also, not every mRNA is reverse-transcripted. So it is more complicated.