TOOLS Easy Fast RT Kit

TOOLS Easy Fast RT Kit is designed for fast first strand cDNA synthesis with high efficiency and able to rapidly remove genomic DNA (gDNA). The kit contains RT Enzyme, RNase Inhibitor, Random primers, Oligo dT primer, dNTP Mixture and reaction buffer. It takes only 15 min (42°C) to complete the synthesis of first strand cDNA by using the innovative RT enzyme. This product also has a high affinity for RNA, which enables efficient and sensitive reverse transcription of any template, such as GC rich and complicated secondary structures of template RNA, leading to high yields of cDNA.

 

No. Size Price Qty
KRT-BA18 100preps $500.00
The price does not include shipping fee and tax. ORDER
Features
  • Complete cDNA synthesis in 15 minutes
  • Directly remove gDNA
  • The kit only contains 1 bottle
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Work Flow

 


  1. Wang WH, Yeh CS, Lin CY, Yuan RY, Urbina AN, Lu PL, Chen YH, Chen YA, Liu FT, Wang SF. Amino Acid Deletions in p6Gag Domain of HIV-1 CRF07_BC Ameliorate Galectin-3 Mediated Enhancement in Viral Budding. Int J Mol Sci. 2020 Apr 21;21(8):2910. 

  2. Tsai TH, Yang CC, Kou TC, Yang CE, Dai JZ, Chen CL, Lin CW. Overexpression of GLUT3 promotes metastasis of triple-negative breast cancer by modulating the inflammatory tumor microenvironment. J Cell Physiol. 2021 Jun;236(6):4669-4680.

  3. Lee KY, Kuo TC, Chou CM, Hsu WJ, Lee WC, Dai JZ, Wu SM, Lin CW. Upregulation of CD109 Promotes the Epithelial-to-Mesenchymal Transition and Stemness Properties of Lung Adenocarcinomas via Activation of the Hippo-YAP Signaling. Cells. 2020 Dec 25;10(1):28.

  4. Wu SC, Chang CH, Chang LH, Wu CW, Chen JW, Chen CH, Lin YS, Chang JK, Ho ML. Simvastatin Enhances the Chondrogenesis But Not the Osteogenesis of Adipose-Derived Stem Cells in a Hyaluronan Microenvironment. Biomedicines. 2021 May 17;9(5):559.

  5. Chang LH, Chen CH, Wu SC, Chang JK, Ho ML. Cyclooxygenase-2 regulates PTHrP transcription in human articular chondrocytes and is involved in the pathophysiology of osteoarthritis in rats. J Orthop Translat. 2021 Aug 12;30:16-30.

Q1. Why can you add gDNA erasor in you mix and still synthesize cDNA? Wont the erasor delete cDNA?
A: The concentration of gDNA erasor is relatively low in the mix so the erasor will not delete cDNA.

Q2. What is the efficiency of Tools Easy Fast RT Kit?
A: Normally, 1ug RNA will have over 90% RT efficiency.