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ToolsQuant II Fast RT Kit

Data sheet MSDS
ToolsQuant II Fast RT Kit is designed for the first strand cDNA synthesis with high efficiency and able to rapidly remove genomic DNA (gDNA). To avoid the contamination of gDNA, the kit contains gDNA Eraser that effectively remove gDNA (42°C, 3 min). It takes only 15 min (42°C) to complete the synthesis of first strand cDNA by using the innovative RT enzyme. This product also has a high affinity for RNA, which enables efficient and sensitive reverse transcription of any template, such as GC rich and complicated secondary structures of template RNA, leading to high yields of cDNA.

 

 

No. Size Price Qty
KRT-BA06-2 100 rxns. $400.00
The price does not include shipping fee and tax. ORDER
Features
  • High efficiency: Reverse transcription efficiency above 95%.
  • gDNA Eraser included：remove genomic DNA in 3min.
  • High performance: Tolerate to GC-rich templates.
  • Fast: Only 21 min experimental procedure.
  • ​Error rate: ~1.0 x10-3
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 Work Flow

                        

 

 

 

 

 

TOOLSQuant II Fast RT Kit Competition Test:


 

  1. Wen YC, Lin YW, Chu CY, Yang YC, Yang SF, Liu YF, Hsiao M, Lee WJ, Chien MH. Melatonin-triggered post-transcriptional and post-translational modifications of ADAMTS1 coordinately retard tumorigenesis and metastasis of renal cell carcinoma. J Pineal Res. 2020 Sep;69(2):e12668. 

  2. Yang WB, Hsu CC, Hsu TI, Liou JP, Chang KY, Chen PY, Liu JJ, Yang ST, Wang JY, Yeh SH, Chen RM, Chang WC, Chuang JY. Increased activation of HDAC1/2/6 and Sp1 underlies therapeutic resistance and tumor growth in glioblastoma. Neuro Oncol. 2020 Oct 14;22(10):1439-1451. 

  3. Huang CS, Ho JY, Chiang JH, Yu CP, Yu DS. Exosome-Derived LINC00960 and LINC02470 Promote the Epithelial-Mesenchymal Transition and Aggressiveness of Bladder Cancer Cells. Cells. 2020 Jun 7;9(6):1419. 

  4. Fang CH, Lin YT, Liang CM, Liang SM. A novel c-Kit/phospho-prohibitin axis enhances ovarian cancer stemness and chemoresistance via Notch3-PBX1 and β-catenin-ABCG2 signaling. J Biomed Sci. 2020 Mar 13;27(1):42. 

  5. Lang YD, Chen HY, Ho CM, Shih JH, Hsu EC, Shen R, Lee YC, Chen JW, Wu CY, Yeh HW, Chen RH, Jou YS. PSPC1-interchanged interactions with PTK6 and β-catenin synergize oncogenic subcellular translocations and tumor progression. Nat Commun. 2019 Dec 16;10(1):5716. 

  6. Li CH, Chang YC, Hsiao M, Liang SM. FOXD1 and Gal-3 Form a Positive Regulatory Loop to Regulate Lung Cancer Aggressiveness. Cancers (Basel). 2019 Nov 28;11(12):1897. 

  7. Huang HC, Chao CC, Wu PH, Chung HY, Lee HY, Suen CS, Hwang MJ, Cai BH, Kannagi R. Epigenetic silencing of the synthesis of immunosuppressive Siglec ligand glycans by NF-κB/EZH2/YY1 axis in early-stage colon cancers. Biochim Biophys Acta Gene Regul Mech. 2019 Feb;1862(2):173-183. 

  8. Hsu RM, Zhong CY, Wang CL, Liao WC, Yang C, Lin SY, Lin JW, Cheng HY, Li PY, Yu CJ. Golgi tethering factor golgin-97 suppresses breast cancer cell invasiveness by modulating NF-κB activity. Cell Commun Signal. 2018 Apr 27;16(1):19. 

  9. Chang CJ, Lu CC, Lin CS, Martel J, Ko YF, Ojcius DM, Wu TR, Tsai YH, Yeh TS, Lu JJ, Lai HC, Young JD. Antrodia cinnamomea reduces obesity and modulates the gut microbiota in high-fat diet-fed mice. Int J Obes (Lond). 2018 Feb;42(2):231-243. 

  10. Wu YY, Hou BH, Lee WC, Lu SH, Yang CJ, Vaucheret H, Chen HM. DCL2- and RDR6-dependent transitive silencing of SMXL4 and SMXL5 in Arabidopsis dcl4 mutants causes defective phloem transport and carbohydrate over-accumulation. Plant J. 2017 Jun;90(6):1064-1078. 

  11. Chang CJ, Lin CS, Lu CC, Martel J, Ko YF, Ojcius DM, Tseng SF, Wu TR, Chen YY, Young JD, Lai HC. Ganoderma lucidum reduces obesity in mice by modulating the composition of the gut microbiota. Nat Commun. 2015 Jun 23;6:7489. Erratum in: Nat Commun. 2017 Jul 11;8:16130. 

  12. Hou CY, Lee WC, Chou HC, Chen AP, Chou SJ, Chen HM. Global Analysis of Truncated RNA Ends Reveals New Insights into Ribosome Stalling in Plants. Plant Cell. 2016 Oct;28(10):2398-2416. 

  13. Yang ZS, Huang SW, Wang WH, Lin CY, Wang CF, Urbina AN, Thitithanyanont A, Tseng SP, Lu PL, Chen YH, Wang SF. Identification of Important N-Linked Glycosylation Sites in the Hemagglutinin Protein and Their Functional Impact on DC-SIGN Mediated Avian Influenza H5N1 Infection. Int J Mol Sci. 2021 Jan 13;22(2):743.

  14. Chu CY, Lee YC, Hsieh CH, Yeh CT, Chao TY, Chen PH, Lin IH, Hsieh TH, Shih JW, Cheng CH, Chang CC, Lin PS, Huang YL, Chen TM, Yen Y, Ann DK, Kung HJ. Genome-wide CRISPR/Cas9 knockout screening uncovers a novel inflammatory pathway critical for resistance to arginine-deprivation therapy. Theranostics. 2021 Jan 25;11(8):3624-3641.

  15. Wu YS, Ho JY, Yu CP, Cho CJ, Wu CL, Huang CS, Gao HW, Yu DS. Ellagic Acid Resensitizes Gemcitabine-Resistant Bladder Cancer Cells by Inhibiting Epithelial-Mesenchymal Transition and Gemcitabine Transporters. Cancers (Basel). 2021 Apr 22;13(9):2032.

  16. ​Huang SW, Shen MC, Wang WH, Li WY, Wang JH, Tseng CY, Liu PY, Wang LS, Lee YL, Chen YA, Lee CY, Lu PL, Wang SF. High prevalence of HIV-1 transmitted drug resistance and factors associated with time to virological failure and viral suppression in Taiwan. J Antimicrob Chemother. 2021 Oct 14:dkab361.

  17. Fang JY, Chou WL, Lin CF, Sung CT, Alalaiwe A, Yang SC. Facile Biofilm Penetration of Cationic Liposomes Loaded with DNase I/Proteinase K to Eradicate Cutibacterium acnes for Treating Cutaneous and Catheter Infections. Int J Nanomedicine. 2021;16:8121-8138

  18. ​Huang CS, Tsai CH, Yu CP, Wu YS, Yee MF, Ho JY, Yu DS. Long Noncoding RNA LINC02470 Sponges MicroRNA-143-3p and Enhances SMAD3-Mediated Epithelial-to-Mesenchymal Transition to Promote the Aggressive Properties of Bladder Cancer. Cancers (Basel). 2022 Feb 15;14(4):968.

Q1. The ct value has increased after using Quant II.
A: The best amount of RNA for RT is 1ug. We suggest you adjust the concentration of RNA and see if the results has altered.

Q2. The efficiency of cDNA synthesis using Quant II is not good.
A: Please check the length of your target gene. The best length is 4kb.

Q3. Will there be any effect if we skip the 95 degrees for Quant II?
A: The purpose is for the enzyme incativation, so it is necessary.

Q4. What is the formula of the primer of Quant II?
A: The primer inculeds random primer and oligo dT, which is 1:1.

Q5. Can Quant II be used for the RNA of E.coli?
A: Yes. Our primer is random primer and can be used for E.coli system.

Q6. Is RNA imput less than 50ng suitabl for Quant II?
A: Not recommended. Please use at least 50ng according to protocol.

Q7. Can Quant II be used for virus?
A: Yes

Q8. What is the suggested concentration if I only want to use oligo dT or random primer, respectively?
A: We suggest you refer to our protocol. 50pmol with the final volume of 20ul. For specific primer the concentration would be 5pmol.

Q9. Can you skip the gDNA removal step? Can you adjust the volume of buffers?
A: We do not recommend skipping gDNA removal step. We suggest you use the buffer according to protocol.

Q10. Do you have Rnase inhibitor in Quant II?
A: Yes