Cytoplasmic and Nuclear Protein Extraction Kit

This kit supplies a complete set of lysis reagents that enable the separation of nuclear protein and cytoplasmic fractions from cultured cells and tissue. The optimized reagents and protocol allow non-denatured, active proteins to be purified in less than two hours. It is sufficient for extracting 50-100 protein samples, the quantity for each sample is (5-10) x 108 cells (about 50mg).



No. Size Price Qty
BRARZ106 100 assays $183.33
The price does not include shipping fee and tax. ORDER
  • Obtain purified protein in 90 minutes.
  • No ultracentrifugation is required.
  • Cross-contamination of cytosolic proteins into the membrane fractions is about 10%
  • ready-to-use in downstream applications such as NGS, RT-PCR, and qPCR, northern blot, polyA screening, etc
  1. Hsu YH, Chuang HC, Lee YH, Lin YF, Chiu YJ, Wang YL, Wu MS, Chiu HW. Induction of Fibrosis and Autophagy in Kidney Cells by Vinyl Chloride. Cells. 2019 Jun 17;8(6):601. 

  2. Wen HC, Huo YN, Chou CM, Lee WS. PMA inhibits endothelial cell migration through activating the PKC-δ/Syk/NF-κB-mediated up-regulation of Thy-1. Sci Rep. 2018 Nov 2;8(1):16247. 

  3. ​Hsu SH, Chou LF, Hong CH, Chang MY, Tsai CY, Tian YC, Yang HY, Yang CW. Crosstalk between E-Cadherin/β-Catenin and NF-κB Signaling Pathways: The Regulation of Host-Pathogen Interaction during Leptospirosis. Int J Mol Sci. 2021 Dec 4;22(23):13132.

Q1. Do you need to add protease, phosphatase inhibitor in every reagent?
A: Yes. This is the optimal step.