Products

TOOLS 3D Culture Plus Kit

TOOLS 3D Culture Plus Kit is a comprehensive set of reagents to establish the microenvironments for 3D cell culture and related applications. The polymers included in TOOLS 3D Culture Plus Kit can generate hydrogels at fast gelation rate. The full control of biomolecular modifications and gel stiffness could be achieved easily and efficiently with this ready-to-use kit and allows a great variety of cell culture applications.
No. Size Price Qty
TTL-3D48 48 assays $450.00
TTL-3D480 480 assays $1,360.00
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Feature
  1. Scaffold-base matrix formulation ideally mimicking in vivo microenvironment for cells.
  2. Allowing full control of biomolecular modifications and gel stiffness to maintain natural morphology.
  3. The special formulated hydrogel can quickly and stably form culturing matrix.
  4. Easy to collect spheroids with the Dissolving buffer in this kit.

Workflow

TOOLS 3D culture Kit

 

3D culture of polycystic kidney disease cells
 


The WT 9-12 cells were cultured in TOOLS 3D culture Plus Kit. WT 9-12 cells were cultured in hydrogel-based 3D culture materials for 4 weeks. Cells were treated with two different drugs and refreshed medium twice a week. Analysis using F-actin (Green) to observe the spheroid structure. Nucleus stained with DAPI (Blue). Scale bar = 100 um.

Great growth and  integrity of spheroids

Figure 1. HCT116 cells were cultured in 3D with TOOLS 3D Culture Plus kit for day 0, day 3, and day 7. The spheroids grew successfully, and the volumes of spheroids increased following days.

Figure 2. Huh 7  and A549 are cultured in 3D and their spheroids were collected with TOOLS 3D Culture Plus Kit.