TOOLS Epstein-Barr Virus (EBV) miRNA Multiplex RT-qPCR Assay
- Well validated: targeting 44 respect EBV mature microRNAs that are suggested of crucial and potential roles in EBV related disease.
- High accuracy: Combined Reverse transcription with stem-looped primer with TagMan probe specially targeting mature miRNA.
- Easy and fast
we using a serial dilution of synthetic cDNAs as templates in qPCR experiment to examined the #48 EBV primer/probe mix specificity. The synthetic cDNA templates (1X107 -1X101copies) were detected by qPCR reaction. The linear Range and sensitivity fell within the expected range.
Figure 1. High sensitivity with a range of synthetic cDNAs
We examined the #48 EBV primer Specificity using cross-hybridization of assay, using synthetic cDNAs as templates in qPCR experiment for assessing the corresponding qPCR primer/probe mix. Ct values (the threshold set as 0.2) are relevant to the Control Lot. The target signals fell within the expected range. The final assay conditions demonstrated no cross-hybridization of each primer.
Figure 2. Broad linear range and high sensitivity