TOOLSwift PCR Cloning Kit

TOOLSwift PCR cloning kit is designed for fast, and direct cloning of the single or multiple DNA into any vector, regardless of DNA fragment end compatibility. The core component of this kit is 2×TOOLSwift Assembly Mix that fuses DNA insert and linearized vector based on the 15 bp overlap sequence at their ends. The 15 bp overlap sequence can be engineered by designing primers for amplification of desire DNA fragments. During assembly reaction, the DNA insert and linearized vector will be accomplished as a closed loop plasmid by sticky-ends creating, gaps filling, and ends linking at the overlap regions. The product of the assembly reaction can be directly to transform competent cells and then select colonies.

Overview of the assembly method.

CHARACTERISTIC

Features and Benefits

 

    ●   TOOLSwift PCR cloning kit can be used to clone 100-15000 bp DNA fragment assembly.

       The kit achieves oriented-assembly of 1-5 insert into a vector.

       High purity of PCR product is not necessary.

 

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Data

Multiple DNA fragments prepared from one target gene.

1: Fragment 1, 736bp

2: Fragment 2, 755bp

3: Fragment 3, 732bp

※Total size of insert gene 1+2+3=2151 bp

Colony PCR of target gene in p3XFLAG-CMV-7.1

※Total size of insert 1+2+3=2151 bp

Download File

Product No. Size Pirce QTY
TOOLSwift PCR Cloning Kit VIT-BB01 20 rxns. $500.00