chemically-modified siRNA synthesis-in vitro
Biotools provides customized siRNA design and synthesis. We also provide customized siRNA set, which guarantees at least 70% knockdown efficiency.
● Free siRNA design
● Provides multiple chemically modify to choose
● High stability and long effect
● Guarantee at least 70% knockdown efficiency for sets
All you need to do is to provide the gene sequence or NCBI number, we will design and choose the siRNA that has the best knockdown effect. We also have chemically modified siRNA which has longer effect and better efficiency.
|chemically modified siRNA 2OD/4OD/10OD||260 / 316.67 / 733.33|
|Negative control siRNA 1OD||83.33|
|FAM labeled negative control siRNA||117.24|
|Positive control siRNA||83.33|
|Chemically-modified siRNA set||Price(USD)|
| 3 x 2 OD negative control siRNA
1 OD negative control siRNA
1 OD FAM labeled negative control siRNA
1 OD positive control siRNA
Working day not including delivery time: 2 weeks
2. Please confirm if you need chemically modified siRNA
3. Please confirm product specificity (single or set)
Jung-Lin Wu, Hsin-Yi Wu, Kuo-I Lin, et al., Temporal regulationof Lsp1 O-GlcNAcylation and phosphorylation during apoptosis ofactivated B cells. nature communications, 2016.
＊Shu-Yi Yin, Feng-Yi Jian, Ning-Sun Yang, et al., Induction of IL-25 secretion form tumor-associated fibroblasts suppresses mammary tumor metastasis. nature communications, 2016.
A: The sequence is the same, but there will be a chemical modification for the siRNA in our chemically-modified set
Q2. How do you check the off-target effect of siRNA?
A: We will avoid this situation when we first design the sequence but the customer must check
Q3. What is the highest concentration for siRNA? Can we pool the 3 siRNAs together?
A: The most we suggest is 4 times more than our recommendation, and we do not suggest you pool the 3 siRNAs together
Q4. Can you evaluate the knockdown efficiency when designing siRNA sequence?
A: No. The knockdown efficiency depends on the cell type
Q5. Where is the exact location of the chemical modification at siRNA?
A: Depends on what type of modification. For O-Me, it will be on the 2' of every ribonucleotide