TOOLS eSpCas9 (1.1) Protein

  The CRISPR (clustered regularly interspaced short palindromic repeats) system is a prokaryotic adaptive immune system that consists of single guide RNA and Cas9 protein for performing genome editing in many organisms. 

TOOLS eSpCas9 (1.1) protein (K848A/K1003A/R1060A) is a purified nuclease from E coli using Cas9 gene of Streptococcus pyogenes. It is a ready-to-use product containing a nuclear localization signal (NLS) for genome editing experiments resulting site-specific cleavage of double stranded DNA.

CHARACTERISTIC

Quality control

 

 

 

     1ug protein, 7.5% SDS-PAGE

 

 

  Lane 1: Supercoiled plasmid

  Land 2: Linear plasmid cut by Cas9 protein

  0.6% agarose gel

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Data

 

  50nM protein with 60nM sgRNA or 100nM protein
  with 12nM sgRNA
  100ng pGEM plasmid incubation 37
˚C, 1hour
  0.6% agarose gel, 100V, 30min
  Target gene: zebrafish Fak1a

Download File

Product No. Size Pirce QTY
TOOLS eSpCas9 (1.1) Protein TS-cas901-50ug 50 ug $830.00
TOOLS eSpCas9 (1.1) Protein TS-cas901-500 500ug $3,400.00
TOOLS eSpCas9 (1.1) Protein TS-cas901-1mg 1mg $5,400.00