Proteomics – The tendency of post-genomics studies

Since the progression and the success of next-generation sequencing, Proteomics, and functional genomics have become two of the most important research in life science.
Biotools Taiwan uses mass spectrometry as our tool to analyze different types of proteins in different samples at the same time. You can not only find biomarkers with protein quantification but also determine the possible post-translational modification (PTM) site which can be very crucial in drug development.

Our advantages:

• Our technical department has more than 10 years of experience in operating mass spectrometry and handling multiple types of samples (e.g., cell/tissue extracts, serum/plasma, urine, saliva)

• We have a unique online 2D-HPLC system, which requires only a small amount of sample to successfully analyze the peptides through mass spectrometry

• We use the best grade of mass spectrometry (Thermo Orbitrap Elite), which provides the best-analyzed data

• We have the best-customized service and provide assistance in the shortest time
   

What kind of analysis do we provide for Proteomics?
Determine the protein in solution or band-------------------------> Protein Identification Analysis
Determine the sequence of a protein--------------------------------> Protein sequencing
Understand the interaction between proteins---------------------> Protein Interaction Analysis
Determine the concentration of a protein--------------------------> Protein Quantification Analysis
Determine N-glycosylation site of a protein------------------------> Post-Translational Modification Analysis
                                   

Protein Interaction Analysis
The function of a protein is coordinated by multiple protein interactions, so creating an interaction network between proteins can help us understand the composition of a protein complex or the up-and-downstream relationship between different proteins. The most common methods to identify protein interaction is yeast two-hybrid (Y2H) and Affinity-purification mass spectrometry (AP/MS). Y2H can only analyze whether two proteins can interact. AP/MS, on the other hand, analysis the whole interactome and can investigate the relationship between multiple proteins.

Difference between Y2H and AP/MS method

For protein interaction analysis we also have Hydrogen-deuterium exchange (HDX) for epitope mapping. HDX is a method to study protein structure and its dynamic changes. We can identify the protein interaction site, epitope mapping, and protein active site identification...etc. The principle of this method is to soak the protein into deuterium solution, which the hydrogen of the protein will be exchanged with deuterium in a neutral environment, and the speed of hydrogen-deuterium exchange at the surface of the protein is faster than the exchange rate inside the protein, using mass spectrometry to identify the different exchange rates between different peptides will allow us to identify the structure of the protein.

Protein Identification Analysis
In order to understand the protein composition of a sample, we can use mass spectrometry, which can analyze large scale of samples, and the results are more direct than using indirect detecting methods like antibodies, which can be affected by the specificity of the antibody.

 

Protein Sequencing Analysis
Protein sequencing can analyze the composition of the amino acids in a protein efficiently
Due to the increase of sensitivity and efficiency of mass spectrometry, customer now can choose a faster and cheaper way than Edman sequencing
 
Protein Quantification Analysis
For protein quantification analysis, we use Isobaric tags for relative and absolute quantification, which is also known as i-TRAQ. It is a protein quantification method using isotopes to "label" proteins from different samples, which results in different molecular weight with the same proteins in different samples. With this method, you can analyze protein quantification between different samples at the same time

i-TRAQ labeling for protein quantification

Post-Translational Modification Analysis
Protein expression will not only be determined by physiological changes of the cell, post-translational modification (PTM) is also a critical factor. The traditional method of identifying PTM site of a protein is done by antibodies, which will be affected by the specificity of an antibody, and also by a chance that there is no antibody that can bind to the specific PTM site.
Using mass spectrometry method can not only analyze and identify all the possible PTM site of a protein but also it is much more direct than identifying through an antibody, which can be more convincing.
For this service, Biotools not only will provide enrichment of ubiquitination, phosphorylation, and N-linked glycosylation for you to have better analyze results, but we also provide 982 kinds of PTM  sites for you to choose to analyze!!!