Protein sequencing
Protein sequencing can analyze the composition of the amino acids in a protein efficiently
Due to the increase of sensitivity and efficiency of mass spectrometry, customer now can choose a faster and cheaper way than Edman sequencing
Due to the increase of sensitivity and efficiency of mass spectrometry, customer now can choose a faster and cheaper way than Edman sequencing
Protein sequencing can analyze the composition of the amino acids in a protein efficiently
Due to the increase of sensitivity and efficiency of mass spectrometry, customer now can choose a faster and cheaper way than Edman sequencing.
Due to the increase of sensitivity and efficiency of mass spectrometry, customer now can choose a faster and cheaper way than Edman sequencing.
Working day not including delivery time: 2 weeks
(a) Sample type: Purified protein with amino acid sequence
(b) Sample amount: ≥20 pmol (~1 ug for 50 kDa protein)
(c) Sample concentration: ≥2 pmol/uL (~0.1 ug/uL for 50 kDa protein)
(d) Sample purity: ≥50%
(e) Platform: 1DLC, LTQ-Orbitrap MS
(b) Sample amount: ≥20 pmol (~1 ug for 50 kDa protein)
(c) Sample concentration: ≥2 pmol/uL (~0.1 ug/uL for 50 kDa protein)
(d) Sample purity: ≥50%
(e) Platform: 1DLC, LTQ-Orbitrap MS
Please click the link below for the specification of Proteomics service
Sample requirement
Sample requirement
1. Summary report. pdf
2. Protein-peptide report. xlsx
2. Protein-peptide report. xlsx
Q1. Must I provide the protein sequence?
A: Due to the fact that protein sequencing by mass spectrometry method is based on comparison to the database to calculate the amino acid composition, so you must provide the protein sequence.
Q2. What is the difference between protein identification and protein sequencing?
A: The principle of these two services are the same. Due to protein sequencing is to have at least 95% of the target protein sequenced, we will additionally analyze the protein sequence and choose the most suitable enzyme for protein digestion
Q3. Are there any limitations for in-gel samples?
A: The size of in-gel samples should not exceed 3x8x1.5mm. We will charge additionally if the gel size is bigger than our standard. For sequencing analysis, we don't suggest in-gel samples. In-gel samples will effect the efficiency of peptide elution, which leads to lower coverage.
A: Due to the fact that protein sequencing by mass spectrometry method is based on comparison to the database to calculate the amino acid composition, so you must provide the protein sequence.
Q2. What is the difference between protein identification and protein sequencing?
A: The principle of these two services are the same. Due to protein sequencing is to have at least 95% of the target protein sequenced, we will additionally analyze the protein sequence and choose the most suitable enzyme for protein digestion
Q3. Are there any limitations for in-gel samples?
A: The size of in-gel samples should not exceed 3x8x1.5mm. We will charge additionally if the gel size is bigger than our standard. For sequencing analysis, we don't suggest in-gel samples. In-gel samples will effect the efficiency of peptide elution, which leads to lower coverage.
Q4. Is there anything we need to notify for protein sequencing?
A: You must provide the sequence of the protein and the purity must >50%.