Service

Probe Synthesis Service

Tools provide a complete PCR probe synthesis service. We provide more than 200 probe modification for you to choose, including phosphorylation, single or double fluorescent modifications...etc
Features:
1. Fast delivery time (2-3 weeks)
2. High quality
3. The order guideline is simple. We will design the probe sequence for you
4. Over 200 modifications for you to choose
Real-time PCR is a very common tool to detect gene expression. This technique uses PCR to amplify the target gene and gain gene expression information in a very short time. This method has a disadvantage which is the specificity of the primer sometimes is not very good and causes false results.
In order to solve this problem, scientists have developed a series of probes, including Taqman, Molecular Beacons...etc. Using the BHQ method, scientists can solve the low specificity problem with the primer. When the target gene is amplified via PCR, the probe will be released due to 5-3 exonuclease activity of DNA polymerase. The probe will then release a fluorescent signal which will be detected by the qPCR machine. The gene expression will be quantified according to the strength of the fluorescent signal
Tools have developed a high-quality probe synthesis service, with over more than 200 different modifications for the customer to choose, including phosphorylation, single or double fluorescent modifications...etc.

Fluorescent probe-double modification list:


For other modification lists please check our modification list
 


 
double modification
type
modification
purification method
TAMRA    
 5' FAM-3' TAMRA
HPLC
5' HEX-3' TAMRA
HPLC
5' TET-3' TAMRA
HPLC
5' JOE-3' TAMRA
HPLC
5' VIC-3' TAMRA
HPLC
BHQ1
5' FAM-3' BHQ1
HPLC
5' VIC-3' BHQ1
HPLC
5' HEX-3' BHQ1
HPLC
5' JOE-3' BHQ1
HPLC
5' TET-3' BHQ-1
HPLC
BHQ2
5' Cy5-3' BHQ2
HPLC
5' Cy3-3' BHQ2
HPLC
5' ROX-3' BHQ2
HPLC
5' TET-3' BHQ2
HPLC
5' JOE-3' BHQ2
HPLC
5' HEX-3' BHQ2
HPLC
5' TAMRA-3' BHQ2
HPLC
5' Texas Red-3' BHQ2
HPLC
5' Quasar 670-3' BHQ2
HPLC
5' FAM and 3' BHQ2
HPLC
BHQ3
  5' Quasar 670-3' BHQ3   
HPLC
5' CY5-3' BHQ3
HPLC
ECLIPS
5' FAM-3' ECLIPS
HPLC
5' HEX-3'  ECLIPS
HPLC
5' TAMRA-3' Eclipse
HPLC
5' ROX-3' Eclipse
HPLC
5' TET-3' Eclipse
HPLC
5' JOE-3' Eclipse
HPLC
MGB
5' FAM-3' MGB
HPLC
5' HEX-3' MGB
HPLC
5' TET-3' MGB
HPLC
5' JOE-3' MGB
HPLC
5' ROX-3' MGB
HPLC
5' Texas Red-3' MGB
HPLC
5' Quasar 670-3' MGB
HPLC
5' VIC-3' MGB
HPLC
Dabcyl
5' FAM-3' DABCYL
HPLC
5' HEX-3' DABCYL
HPLC
5' TET-3' DABCYL
HPLC
5' JOE-3' DABCYL
HPLC
5' TAMRA-3' DABCYL
HPLC
  5' Texas Red-3' Dabcyl   
HPLC
5' ROX-3' Dabcyl
HPLC
5' CY3-3' DABCYL
HPLC
5' Cy5-3' DABCYL
HPLC
其他
5' Phos and 3' FAM
HPLC
   5' TAMRA and 3' Phos   
HPLC
5' C6-Biotin, 3' Biotin
HPLC
5' C6-Biotin , 3' Cy5
HPLC
   5' C6-NH2 , 3' C3-Fam   
HPLC
5' C6-NH2 , 3' Cy3
HPLC
5' Cy5 , SS ,
HPLC
5' Cy5 , 3' C7-NH2
HPLC
5' Cy5 , 3' SH
HPLC
5' Dabcyl , 3' C3-Fam
HPLC
5' DlG , 3' Digoxin
HPLC
5' SH,3' Cy3
HPLC
5' Fam,3' C3-Fam
HPLC
      5' TAMRA,3' C3-Fam     
HPLC
single modification
type modification
  purification method 
base
dI
PAGE
dU
PAGE
m5C
PAGE
m6A
PAGE
2'-O-Methyl
PAGE
RNA
PAGE
3' -dT
PAGE
DC-Br
PAGE
LNA-X
PAGE
5-Methyl dC
PAGE
  Phosphorothioates  
S
PAGE
Phosphorylation
5' PHO
PAGE
3' PHO
PAGE
Amino Linkers
5' NH2 C6
HPLC
3' NH2 C7
HPLC
5' NH2 C12
HPLC
Thiol
3' SH C3 
HPLC
5' SH C6 
HPLC
Biotin
5' Biotin
HPLC
3' Biotin TEG
HPLC
Digoxigenin
5' DIG
HPLC
3' DIG
HPLC
Fluorescence
5' Cy3
HPLC
3' Cy3
HPLC
5' Cy5
HPLC
3' Cy5
HPLC
5' FAM
HPLC
3' FAM
HPLC
5' HEX
HPLC
5' TET
HPLC
5' -FITC
HPLC
3' -FITC
HPLC
5' 6-JOE
HPLC
3' 6-JOE
HPLC
5' ROX
HPLC
3' ROX
HPLC
5' TAMRA
HPLC
3' TAMRA
HPLC
3' -HEX
HPLC
3' -TET
HPLC
5' Helix Flour555
HPLC
3' Helix Flour555
HPLC
5' Helix Flour575
HPLC
3' Helix Flour575
HPLC
5' Texas Red
HPLC
3' Texas Red
HPLC
5' Quasar 570
HPLC
3' Quasar 570
HPLC
5' Quasar 670
HPLC
3' Quasar 670
HPLC
5' Cy3.5
HPLC
3' Cy3.5
HPLC
5' Cy5.5NS
HPLC
3'Cy5.5NS
HPLC
5' Cy7NS
HPLC
3' Cy7NS
HPLC
    5' & 3' CAL Fluor Red 590    
HPLC
  5' & 3' CAL Fluor Red 610  
HPLC
5' & 3' DABCYL
HPLC
5' & 3' AMCA
HPLC
5' & 3' DEAC
HPLC
5' & 3' MCA 
HPLC
5' & 3' LRB Red 
HPLC
5' & 3' CR6G
HPLC
     5' & 3' Bodi Fluor R6G    
HPLC
5' & 3' NBD-X
HPLC
5' & 3' California Red
HPLC
5' & 3' iFlour
HPLC
5' -VIC
HPLC
3' -LC RED-610
HPLC
3' -LC RED-640
HPLC
3' -LC RED-705
HPLC
3' -Bodipy 493/503
HPLC
3' -Bodipy 564/570
HPLC
3' -Bodipy 581/591
HPLC
3' -Bodipy 630/650
HPLC
3' -Bodipy 650/665
HPLC
3 -BODIPY R6G
HPLC
5' -SIMA
HPLC
(Quenchers)
3' DABCYL
HPLC
3' TAMRA
HPLC
3' BHQ 1
HPLC
3' BHQ 2
HPLC
3' BHQ 3
HPLC
3' -MGB
HPLC
3' BBQ-650
HPLC
3' TQ1-TQ6
HPLC
3' -QSY 7 carboxylic acid
HPLC
3' TQ7
HPLC
3' eclipse
HPLC
其他修飾
Spacer9
HPLC
Azide
HPLC
DBCO
HPLC
Spacer(C6)
HPLC
Spacer9
HPLC
Spacer(C12)
HPLC
Spacer C3
HPLC
Spacer C18
HPLC
dSpacer
HPLC
Inverted Base
HPLC
5' -Carboxy C10
HPLC
5' -D4PA
HPLC
5' -Acridine
HPLC
3' ddC
HPLC
Alexa Flour 647
HPLC
Yakima Yellow Epoch
HPLC
Pyrene
HPLC
中間修飾
Int NH2 dT
HPLC
Int Biotin dT
HPLC
Int Cy3 dT
HPLC
Int Cy5 dT
HPLC
Int TAMRA dT
HPLC
Int ROX dT
HPLC
Int FAM dT
HPLC
Int HEX dT
HPLC
Int Dig dT
HPLC
Int CHCH dT
HPLC
Int DBCO dT
HPLC
Int Azide dT
HPLC
Int TAMRA dT
HPLC
Int ATTO Flour dT
HPLC
Int BHQ1 dT
HPLC
Int BHQ2 dT
HPLC
Int HS-SH C6
HPLC
Int Alexa Flour dT
HPLC
Int Azobenzene
HPLC
Int Ferrocene dT
HPLC
dT- LC RED-610
HPLC
dT- LC RED-640
HPLC
dT- LC RED-705
HPLC
dC-Fluorescein
HPLC
dC-Tamra
HPLC
dC-Amino
HPLC
dC-Rox
HPLC
dC-Digoxin
HPLC
dC-FITC
HPLC
dC-Texas Red
HPLC
  • Please provide the correct sequence
  • Select modification and purification method
  • Please confirm the quantity of your order
  • Provide the synthetic probe according to your specification
  • Synthetic report