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HE Swift Cloning Kit

Data sheet MSDS
TOOLS HE Swift Cloning Kit is an advanced positive selection system for the highest efficiency cloning of PCR products generated with Pfu DNA polymerase, Taq DNA polymerase, or other thermo-stable DNA polymerases. Additionally, any other DNA fragment, either blunt or sticky-end, can be successfully cloned using the kit. Cloning is fast and efficient; ligation takes only 5 minutes and yields more than 99% positive clones.
The kit features the novel positive selection cloning vector pHE. This vector contains a lethal gene which is disrupted by ligation of a DNA insert into the cloning site. As a result, only cells with recombinant plasmids are able to propagate, eliminating the need for expensive blue/white screening.
The vector contains an expanded multiple cloning site, as well as a T7 promoter for in vitro transcription. Sequencing primers are included for convenient sequencing of the insert.


Cloning Principle
Quick and efficient: Blue/white screening is not necessary, and the whole ligation process can be finished within 5 min.
Sensitive and widely applied: Good for the ligation of low concentration fragments and long fragments, works for both blunt and sticky end product.

No. Size Price Qty
VTT-BB05 20 rxns. $250.00
The price does not include shipping fee and tax. ORDER
Features
  • No need for blue white screening.
  • Ligation time: 5 minutes.
  • Suitable for blunt-end and sticy-end PCR product.

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pHE Vector Map:

 

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Work Flow

 

 

 Insert PCR Product from HE Swift Cloning Kit

Fig 1. Insert product (size: 909 bp).
M, Marker; Lane 1, Insert PCR product.
 

Ligation Mixture PCR from HE Swift Cloning Kit

Fig 2. Ligation mixture
M, Marker; Lane 1, ligation mixture
 

 Colony PCR determining positive clone from HE Swift Cloning Kit

Fig 3. Colony PCR

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  2. Chang WM et al. "AKR1C1 controls cisplatin-resistance in head and neck squamous cell carcinoma through cross-talk with the STAT1/3 signaling pathway." Journal of Experimental & Clinical Cancer Research 38.1 (2019): 245.

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  4. PENG SY et al. miR‐134 targets PDCD7 to reduce E‐cadherin expression and enhance oral cancer progression. International journal of cancer, 2018, 143.11: 2892-2904.

  5. Yeh TH, Liu HF, Chiu CC, Cheng ML, Huang GJ, Huang YC, Liu YC, Huang YZ, Lu CS, Chen YC, Chen HY, Cheng YC. PLA2G6 mutations cause motor dysfunction phenotypes of young-onset dystonia-parkinsonism type 14 and can be relieved by DHA treatment in animal models. Exp Neurol. 2021 Dec;346:113863.

Q1. What is the recommend competent cell for HE Cloning kit?
A: Typical competent cell is suitable.

Q2. How to improve the efficiency of HE Swift cloning kit?
A: Every step of cloning can increase efficiency. Whether insert and vector has been purified, the  propoion of vector and insert. The protocol for transformation is also inportant.