TOOLS 2xSYBR qPCR Mix Kit is specially designed to perform Real-time PCR in SYBR Green I fluorescent-based detection assays. The Real-Time PCR reaction buffer, a 2× pre-mixed solution included in this kit, provides an optimum concentration of SYBR Green I solution, which greatly facilitates the preparation of qPCR reaction mixture. TOOLS 2xSYBR qPCR Mix adopts a unique dual hot-start enzymes system (chemically modified HotStar Taq DNA polymerase and antibody modified Anti Taq DNA Polyerase), which, plus the pre-optimized buffer solution, provides a convenient format for highly sensitive and specific qPCR amplification.


No. Size Price Qty
FPT-BB05 1.25ml $50.00
The price does not include shipping fee and tax. ORDER
  • Unique dual hot-start enzymes system
  • Simple and convenient
  • ROX dye in included seperately

​Efficiency and stability in various sample concentration

Tools 2x SYBR qPCR Mix Efficiency Test

  1. Hsieh CH, Chu CY, Lin SE, Yang YSH, Chang HS, Yen Y. TESC Promotes TGF-α/EGFR-FOXM1-Mediated Tumor Progression in Cholangiocarcinoma. Cancers (Basel). 2020 Apr 29;12(5):1105. 

  2. Chen CY, Chen CC, Chuang WY, Leu YL, Ueng SH, Hsueh C, Yeh CT, Wang TH. Hydroxygenkwanin Inhibits Class I HDAC Expression and Synergistically Enhances the Antitumor Activity of Sorafenib in Liver Cancer Cells. Front Oncol. 2020 Feb 25;10:216. 

  3. Huang CS, Ho JY, Chiang JH, Yu CP, Yu DS. Exosome-Derived LINC00960 and LINC02470 Promote the Epithelial-Mesenchymal Transition and Aggressiveness of Bladder Cancer Cells. Cells. 2020 Jun 7;9(6):1419. 

  4. Li CJ, Lin HY, Ko CJ, Lai JC, Chu PY. A Novel Biomarker Driving Poor-Prognosis Liver Cancer: Overexpression of the Mitochondrial Calcium Gatekeepers. Biomedicines. 2020 Oct 24;8(11):E451. 

  5. Shao PL, Wu SC, Lin ZY, Ho ML, Chen CH, Wang CZ. Alpha-5 Integrin Mediates Simvastatin-Induced Osteogenesis of Bone Marrow Mesenchymal Stem Cells. Int J Mol Sci. 2019 Jan 24;20(3):506. 

  6. Lin EY, Bayarsengee U, Wang CC, Chiang YH, Cheng CW. The natural compound 2,3,5,4'-tetrahydroxystilbene-2-O-β-d glucoside protects against adriamycin-induced nephropathy through activating the Nrf2-Keap1 antioxidant pathway. Environ Toxicol. 2018 Jan;33(1):72-82. 

  7. Wu SC, Huang PY, Chen CH, Teong B, Chen JW, Wu CW, Chang JK, Ho ML. Hyaluronan microenvironment enhances cartilage regeneration of human adipose-derived stem cells in a chondral defect model. Int J Biol Macromol. 2018 Nov;119:726-740. 

  8. Hsieh YC, Chiang MC, Huang YC, Yeh TH, Shih HY, Liu HF, Chen HY, Wang CP, Cheng YC. Pparα deficiency inhibits the proliferation of neuronal and glial precursors in the zebrafish central nervous system. Dev Dyn. 2018 Dec;247(12):1264-1275. 

  9. Chen CY, Fang JY, Chen CC, Chuang WY, Leu YL, Ueng SH, Wei LS, Cheng SF, Hsueh C, Wang TH. 2-O-Methylmagnolol, a Magnolol Derivative, Suppresses Hepatocellular Carcinoma Progression via Inhibiting Class I Histone Deacetylase Expression. Front Oncol. 2020 Aug 11;10:1319.

  10. ​Chu CY, Lee YC, Hsieh CH, Yeh CT, Chao TY, Chen PH, Lin IH, Hsieh TH, Shih JW, Cheng CH, Chang CC, Lin PS, Huang YL, Chen TM, Yen Y, Ann DK, Kung HJ. Genome-wide CRISPR/Cas9 knockout screening uncovers a novel inflammatory pathway critical for resistance to arginine-deprivation therapy. Theranostics. 2021 Jan 25;11(8):3624-3641.

  11. Wu YS, Ho JY, Yu CP, Cho CJ, Wu CL, Huang CS, Gao HW, Yu DS. Ellagic Acid Resensitizes Gemcitabine-Resistant Bladder Cancer Cells by Inhibiting Epithelial-Mesenchymal Transition and Gemcitabine Transporters. Cancers (Basel). 2021 Apr 22;13(9):2032.

  12. Li CJ, Lin LT, Chu PY, Chiang AJ, Tsai HW, Chiu YH, Huang MS, Wen ZH, Tsui KH. Identification of Novel Biomarkers and Candidate Drug in Ovarian Cancer. J Pers Med. 2021 Apr 19;11(4):316.

  13. Li CJ, Chiu YH, Chang C, Chang YI, Sheu JJ, Chiang AJ. Acetyl Coenzyme A Synthase 2 Acts as a Prognostic Biomarker Associated with Immune Infiltration in Cervical Squamous Cell Carcinoma. Cancers (Basel). 2021 Jun 22;13(13):3125.

  14. Wu SC, Chang CH, Chang LH, Wu CW, Chen JW, Chen CH, Lin YS, Chang JK, Ho ML. Simvastatin Enhances the Chondrogenesis But Not the Osteogenesis of Adipose-Derived Stem Cells in a Hyaluronan Microenvironment. Biomedicines. 2021 May 17;9(5):559.

  15. Chang LH, Chen CH, Wu SC, Chang JK, Ho ML. Cyclooxygenase-2 regulates PTHrP transcription in human articular chondrocytes and is involved in the pathophysiology of osteoarthritis in rats. J Orthop Translat. 2021 Aug 12;30:16-30.

  16. ​Li CJ, Lin LT, Tsui KH. Dehydroepiandrosterone Shifts Energy Metabolism to Increase Mitochondrial Biogenesis in Female Fertility with Advancing Age. Nutrients. 2021 Jul 17;13(7):2449.

  17. ​Chen CC, Chen CY, Cheng SF, Shieh TM, Leu YL, Chuang WY, Liu KT, Ueng SH, Shih YH, Chou LF, Wang TH. Hydroxygenkwanin Increases the Sensitivity of Liver Cancer Cells to Chemotherapy by Inhibiting DNA Damage Response in Mouse Xenograft Models. Int J Mol Sci. 2021 Sep 9;22(18):9766.

  18. Fang JY, Chou WL, Lin CF, Sung CT, Alalaiwe A, Yang SC. Facile Biofilm Penetration of Cationic Liposomes Loaded with DNase I/Proteinase K to Eradicate Cutibacterium acnes for Treating Cutaneous and Catheter Infections. Int J Nanomedicine. 2021;16:8121-8138 
  19. ​Wen CY, Tsui KH, Chang CH, Chiu YH, Lin SA, Chu CY, Li CJ. Integrative Multiomics Evaluation of IIDH1 Metabolic Enzyme as a Candidate Oncogene That is Correlated with Poor Prognosis and Immune Infiltration in Prostate Adenocarcinoma. J Oncol. 2022 Jan 29;2022:9854788.

  20. Huang CS, Tsai CH, Yu CP, Wu YS, Yee MF, Ho JY, Yu DS. Long Noncoding RNA LINC02470 Sponges MicroRNA-143-3p and Enhances SMAD3-Mediated Epithelial-to-Mesenchymal Transition to Promote the Aggressive Properties of Bladder Cancer. Cancers (Basel). 2022 Feb 15;14(4):968
  21. ​Chu PY, Tzeng YT, Tsui KH, Chu CY, Li CJ. Downregulation of ATP binding cassette subfamily a member 10 acts as a prognostic factor associated with immune infiltration in breast cancer. Aging (Albany NY). 2022 Mar 5;14(5):2252-2267

  22. Liu WC, Chuang HC, Chou CL, Lee YH, Chiu YJ, Wang YL, Chiu HW. Cigarette Smoke Exposure Increases Glucose-6-phosphate Dehydrogenase, Autophagy, Fibrosis, and Senescence in Kidney Cells In Vitro and In Vivo. Oxid Med Cell Longev. 2022 Mar 27;2022:5696686.
  23. Shen PC, Lu CC, Chou SH, Liu ZM, Su SJ, Tien YC. Zonal-Layered Chondrocyte Sheets for Repairment of Full-Thickness Articular Cartilage Defect: A Mini-Pig Model. Biomedicines. 2021 Nov 30;9(12):1806.

Q1. Can 2x SYBR be used on machines without ROX dye?
A: Yes.

Q2. Do you need to add ROX dye for ABI ViiA7?
A: Yes.