Products

TOOLS eSpCas9 (1.1) Protein

The CRISPR (clustered regularly interspaced short palindromic repeats) system is a prokaryotic adaptive immune system that consists of single guide RNA and Cas9 protein for performing genome editing in many organisms. 

TOOLS eSpCas9 (1.1) protein (K848A/K1003A/R1060A) is a purified nuclease from E coli using Cas9 gene of Streptococcus pyogenes. It is a ready-to-use product containing a nuclear localization signal (NLS) for genome editing experiments resulting site-specific cleavage of double stranded DNA.

No. Size Price Qty
TS-cas901-50ug 50 ug $830.00
TS-cas901-500 500ug $3,400.00
TS-cas901-1mg 1mg $5,400.00
The price does not include shipping fee and tax. ORDER
Features
  • High purity & efficiency
  • High specificity
  • No off target
  • Suitable for cell and animal experiment
 

 

Protein Purity Test:

1ug protein, 7.5% SDS-PAGE


Protein Activity Test:

          

Lane 1: Supercoiled plasmid
Land 2: Linear plasmid cut by Cas9 protein
             0.6% agarose gel

 

50nM protein with 60nM sgRNA or 100nM protein
with 12nM sgRNA 100ng pGEM plasmid incubation
37˚C, 1hour 0.6% agarose gel, 100V, 30min
Target gene: zebrafish Fak1a

 

  

Q1. Can you aliquote eSpCas9(1.1)? Will the effect be altered?
A: Yes you can aliquote. Please use the products within 1-2 days.