TOOLS Absolute Human Telomere Length qPCR assay Kit
TOOLS Absolute Human Telomere Length qPCR Assay Kit is engineered for the precise quantitative determination of average human telomere length using a robust SYBR Green qPCR platform. By simultaneously measuring telomeric DNA repeats alongside a single-copy reference gene (SCG) and incorporating a pre-calibrated Human Reference DNA standard, this assay delivers accurate absolute quantification in base pairs .
- Direct Absolute Quantification：Includes a calibrated Human Reference DNA standard to calculate absolute telomere length (kbp/diploid cell or kbp/chromosome end).
- High Precision & Reproducibility：Achieves low variation (CV < 6%) and superior run-to-run correlation (R2 = 0.9519).
- Optimal PCR Efficiency：Reaches high PCR efficiency (TEL E = 101.2%；SCG E = 99.1%) with excellent linearity (R2 = 0.999).
- Low Sample Input：Requires only 1-2.5 ng of human gDNA per reaction.
- Clean Signal：Utilizes hot-start DNA polymerase to minimize primer dimers and non-specific amplification.
- Fast Workflow：Delivers high-throughput analysis in under 2 hours.
- Performance of TEL Quantification：
- Performance of SCG Quantification：
- Superior Run-to-Run Consistency of TOOLS Absolute Assay：
