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TOOLS Cell Counting (CCK-8) kit

TOOLS Cell Counting (CCK-8) kit is a ready-to-use solution without premixing of components. Cell Counting Kit-8, being nonradioactive, allows sensitive colorimetric assays for the determination of the number of viable cells in cell proliferation and cytotoxicity assays. WST-8 is reduced by dehydrogenases in cells to give a yellow colored product (formazan), which is soluble in the tissue culture medium. The amount of the formazan dye generated by the activity of dehydrogenases in cells is directly proportional to the number of living cells. The detection sensitivity of CCK-8 is higher than other tetrazolium salts such as MTT, XTT, MTS or WST-1.

 

 

No. Size Price Qty
TEN-CCK8 500 assays $160.00
The price does not include shipping fee and tax. ORDER
Features
  • One-bottle Solution: no premixing of components is required.
  • Lower Toxicity: cells can be use for others experiments after CCK-8 assay.
  • Hight Sensitivity: CCK-8 is the highest sensitive dye for cell-based assay.
  • Many Applications: Can be used on cell proliferation, cell toxicity, drug selection experiments.
  • Data Repeatability: results of the data has been confirmed many times and can be repeated.
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Theory

    

 

 

Comparison between CCK-8 and other cell proliferation /cytotoxicity assays.
Method
MTT
XTT
WST-1
CCK-8
Sensitivity
Low
Medium
Medium
High
Stability
Medium
Low
Medium
High
Cytotoxicity
High
Low
Low
Lower
Procedure Time
Long 
Short
Short
Lower
Detecting Time
560-600nm
420-480nm
420-480nm
430-490nm


Comparison of sensitivity between CCK-8 and MTT test



 
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  • Hsiao, Sung-Han, et al. "The positive inotropic agent DPI-201106 selectively reverses ABCB1-mediated multidrug resistance in cancer cell lines." Cancer letters 434 (2018): 81-90.