RNAi Synthesis Service

   RNA interference (RNA interfering,RNAi) phenomenon was first unknowningly observed when RNA was shown to inhibit protein expression in plants and fungi by process,then known respectively as post-transcriptional gene silencing and quelling. In 1998,Fire and Mello first observed that double-stranded RNA was the source of sequence-specific protein inhibition in C.elegans known as RNA interference. While the studies in C.elegans were encouraging, RNAi was limited in use to lower organisms because delivering long dsRNA for RNAi was non-specifically inhibitory in mammalian cells. Further studies in plants and invertebrate animals demonstrated that actual molecules that lead to RNAi were short double-stranded RNA oligonucleotides,21 to 22 nucleotides in length, processed internally by an enzyme called Dicer. The Dicer cleaverage products are referred to as short (or small)interfering RNA and are today popularly known as siRNA.

   RNA interference (RNAi) is the best way to effectively knock down gene expression to study protein function in a wide range of cell types. Traditional RNAi methods for gene knockdown in mammalian cells involved the use of synthetic RNA duplexes consisting of two unmodified 21-mer oligonucleotides annealed together to form short/small interfering RNAs (siRNAs).

   TOOLS Chemically-Modified siRNA results in greater longevity in cell culture and stability in cell culture and in serum,enhancing the ability for this to be used for in vitro or in vivo aplications and has much more extentional effective time than Standdard siRNA






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