Custom Cloning & Sub-cloning Service

 

  TOOLS provides custom cloning and sub-cloning services to free your energy and time from routine subcloning for more creative research. Our subcloning service is highly flexible to any specific project requirements.

 

TOOLS Cloning & Sub-Cloning Service Procedure :  

 

 

TOOLS Provide two types of Cloning & Sub-Cloning Service :

 

Vectors List You Can Choice :

1.  E. Coli. Expressing Vectors

     (1) pGEX-4T-1   (2) pET30a   (3) pMAL-p5x

     (4) pUC19   (5) pTEF1/Zeo

 

2.  Mammalian Expressing Vectors

     (1) p3XFLAG-myc-CMV26                              (2) p3XFLAG-myc-CMV-7.1

     (3) p3XFLAG-myc-CMV-8                               (4) pCDNA3.1(+)

     (5) pCDNA3.1(-)                                              (6) pLenti-GIII-GFP-2A-Puro(human)

     (7) pIRES2-EGFP                                            (8) pIRES-hrGFP-2a

     (9) pEGFP-N2                                                 

     (11) pLenti-GIII-CMV-GFP-2A-Puro(mouse)   (12) pLenti-His

     (13) pCMV6                                                     (14) pCMV-EGFP

     (15) pCD516B                                                 (16) pLVX-IRES-puro

     (17) pmcharry-N1

 

3.  Yeast Expressing Vectors

     (1) pYES2                        (2) pGAD GH

     (3) pYC2/CT                    (4) pTEF1/Zeo

 

4. Reporter Expression

    (1) pVIVO2-GFP/LacZ              (2) pGL3

    (3) pGL4                                   (4) pGL4.17

    (5) pMIR-reporter Luciferase    (6) pMIR-reporter beta-gal control

 

Delivery Specifications : 

  Both of our Custom Cloning services guarantee 100% sequence fidelity and deliver everything you need to put your new constructs to work right away. Our delivery package includes the following:

     1. 5 μg destination vector containing the gene of your interest

     2. Sequence report covering your gene

     3. Construct map for the plasmid

     4. Data sheet

 

Related Service : 

Gene Synthesis 

 

Quotations and Ordering : 

For quotation requests and questions, you may contact us by phone, email, fax, or via our online quotation system.