TOOLSignal boosting buffer

TOOLSignal Boosting Buffer is the best alternative for conventional blocking buffer and it improves the performance of your Western blot experiments for all types of antibodies. Simply incubate this buffer with primary or secondary antibodies without using any blocking buffer (milk or BSA) to obtain effective blocking result. TOOLSignal Boosting Buffer shortens experimental time and reduces the use of antibodies without compromising Western Blot signal intensity.

CHARACTERISTIC

Features and Benefits


    ●   No blocking step.

    ●   No background.

       High sensitivity.

       Save time.

       Save antibody.

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  Data 

 

    

     Fig 1. Lane 1: RD cells. Lane 2: Stual1- knockout RD cells.
      Protein input: 30ug.
      
1° antibody: Anti-Stau1 antibody 1:1000.
                          Anti-beta-actin antibody 1:10000.
      2
° antibody: 1:5000.
 

    
      
Fig 2. Cell line: BHK-21.
      1
° antibody: KDEL Receptor (N1C1) 1:1000.
      2
° antibody: Goat anti Rabbit 1:10000.
      Protein input: lane 1,3= 30ug ; lane 2,4= 60ug.

 

   
       Fig 3. Cell line: BHK-21.

       TOOLSignal boosting buffer:
       1
° antibody 1:2000 in buffer, RT 1 hr.
       2
° antibody 1:1000 in buffer, RT 1 hr.
       5% milk:
       blocking 30 min.
       1
° antibody 1:2000 in PBST, RT 1 hr.
       2
° antibody 1:1000 in PBST, RT 1 hr.

 

 

 

 


 

        
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  Work Flow
 

                       

Product No. Size Pirce QTY
TOOLSignal boosting buffer TW-NB250 250ml $140.00